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Bacillus Subtilis DE111 Polyphenol Probiotic Drink

A polyphenol-rich probiotic juice made with cold-pressed fruit and fermented with Bacillus subtilis DE111, a clinically studied spore-forming probiotic strain. This 72-hour controlled fermentation supports microbiome balance and functional gut ecology.
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Equipment

  • (1000-1200 mL capacity) Glass jar
  • 1 Cold press juicer (preferred) or blender + strainer
  • 1 Large pot (for hot water bath)
  • 1 Kitchen thermometer
  • 1 Alcohol wipes or food-grade sanitizer
  • 1 Non-metal spoon (sterilized)
  • 1 Incubator / Sous vide / Yogurt maker / Warm oven (37°C setting)

Ingredients
  

  • 2 capsules Bacillus subtilis DE111 (approx. 10 billion CFU)
  • 3 pomegranates, 3 dragon fruits (pink preferred)/ 2 kiwi fruits, 10 strawberries (Can choose any two Polyphenol rich fruits. Cold press to yield 750–800 mL juice)
  • 2 tsp Pectin/Dextrose, GOS, Inulin of each Now food, GOS from Bimuno

Instructions
 

  • Cold press selected fruits to obtain 750–800 mL juice.If using blender, strain pulp thoroughly.
  • Add pectin/dextrose, GOS, Inulin and mix evenly.
  • Place jar in a pot of water. Sous vide is more convenient to use.
  • Heat water to 90°C and maintain for 5 minutes.
  • Turn off heat and allow juice to cool completely to room temperature (≤37°C).
  • Turn off fans/AC and close windows. Wash hands or wear gloves.Wipe counter, jar exterior, and probiotic bottle with alcohol.Allow surfaces to air dry.
  • Open 2 capsules of DE111 into cooled juice.
  • Stir gently with sterilized stainless steel spoon or shake jar to mix evenly.
  • Incubate at 37°C (98°F).Ferment for 72 hours. Can do 60 hours if its very sour.
  • After fermentation, seal jar and refrigerate immediately.
  • Can be consumed after 3-4 hours of storage, use 2-3 tbsp of this juice as starter to ferment next batch.

Notes

  • The final juice will taste mildly tart with light effervescence.
  • The product will not thicken like yoghurt.
  • Discard if a strong alcoholic smell or visible mould develops.
  • Refrigerate and consume within 2 weeks.

Serving

  • 50–60 mL daily
  • Can be taken anytime
  • Shake gently before use
DE111(Brand Leafstar) is a heat-resistant, shelf-stable Bacillus subtilis probiotic strain that survives stomach acid to support digestive balance, nutrient absorption, and immune signalling. Can be purchased from Amazon Europe, U.K
Benefits Include:
  • Digestive comfort & regularity
  • Microbiome balance
  • Immune support pathways
  • Stable & viable through the GI tract
  • Does not require refrigeration

How This Works

Bacillus subtilis DE111 is a spore-forming probiotic that:
  • Survives stomach acid
  • Germinates in the small intestine
  • Supports digestive balance
  • Supports immune signalling through the gut

Signs of Proper Preparation

  • Even pink colour if using dragon fruit
  • No sour smell
  • Discard if unusual odour or visible contamination develops.

Where to Source DE111

Look for supplements labeled specifically:
Bacillus subtilis DE111® (5 Billion CFU per capsule)
Available via:
  • Amazon UK
  • Amazon EU (Belgium, Germany, etc.)
  • Speciality probiotic retailers
  • Brands such as Pro-Subtilis (check label for DE111® trademark)
Always confirm the label lists DE111® — strain specificity matters.
https://www.gosupps.com/pro-subtilis-bacillus-subtilis-de111-60-capsules-5-billion-cfu-per-capsule.html
https://www.amazon.com.be/-/en/Pro-subtilis-Bacillus-subtilis-DE111-capsules/dp/B0C7PNFZ36

Bacillus subtilis DE111 Benefits & Related Scientific Evidence

Benefits for kids: